Blue native PAGE resolution of renal sodium transporters

Analysis of membrane protein complexes by blue native PAGE.

Blue native polyacryamide gel electrophoresis is a special case of native electrophoresis for high resolution separation of enzymatically active protein complexes from tissue homogenates and cell fractions. The method is powerful between 10 and 10,000 kDa. Also membrane protein complexes are separated well after solubilization of complexes with mild neutral detergents. The separation principle ...

The Role of Blue Native/ SDS PAGE in Depression Research

Regulation of renal sodium transporters during severe inflammation.

Sepsis-associated acute renal failure is characterized by decreased GFR and tubular dysfunction. The pathogenesis of endotoxemic tubular dysfunction with failure in urine concentration and increased fractional sodium excretion is poorly understood. This study investigated the regulation of renal sodium transporters during severe inflammation in vivo and in vitro. Injection of high-dosage LPS re...

Central control of renal sodium-phosphate (NaPi-2) transporters.

Regulation of phosphate (Pi) reabsorption occurs through the up- and downregulation of the renal type-II sodium Pi cotransporters (NaPi-2). Recently, renal NaPi2-type expression has been identified in areas of the brain. The present study determined whether brain NaPi-2 is regulated by dietary Pi and whether the behavioral and renal adaptations to low-dietary Pi are controlled centrally. NaPi-2...

Renal Tubular Sodium Transporters and Genetic Basis of Hypertension

Renal regulation of total body sodium (Na) plays an important role in volume-dependent blood pressure control through different molecular mechanisms. Gain of renal tubular Na transporter function in different segments is primarily responsible for essential and monogenic forms of hypertension. In this review, we summarize the physiological regulation of the major renal tubular Na transporters an...